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Faecal occult blood

Faecal occult blood can be a symptom of various diseases. This symptom is also called human occult blood in stool or human haemoglobin. In the early stages of some gastrointestinal disorders, such as colon cancer, ulcers, polyps, colitis, diverticular disease and fissures, there may be no visible symptoms, only occult blood. The Faecal Occult Blood Screening Test is a rapid test for the qualitative detection of low levels of occult blood in faeces.

Sample Collection and Preparation.

Samples should not be collected during, or in the three days prior to the menstrual period, or in cases where the patient has bleeding haemorrhoids or blood in the urine.

Alcohol, aspirin, and other medications taken in excess can cause gastrointestinal irritation and cause occult bleeding. The patient should stop taking these substances at least 48 hours prior to test.

No dietary restrictions are necessary before using the test.

Instructions for use:

Allow the test, sample collection tube, sample and/or controls to reach room temperature (15 – 30 °C) prior to test.

1. Collect the faeces sample in a clean and dry sample collection vessel. The best results will be obtained if the test is carried out within 6 hours of collection. The sample collected may be stored for 3 days at 2 – 8 °C if it is not analysed within 6 hours of collection.

2. To process stool samples, unscrew the stopper from the sample collection tube, then use the sampling stick to randomly puncture the faecal sample in at least 3 different locations. Do not take a stool sample. Screw and tighten the stopper of the sample collection tube and then shake the sample collection tube vigorously to mix the sample and the extraction tampon. Samples prepared in the sample collection tube may be stored for 3 days at room temperature (15-30°C) if not analysed within the hour.

3. Remove the detection device from the sealed container and use it as soon as possible. Do not touch the strip membrane.

4. Keep the sample collection tube upright, then unscrew and open the top cap. Reverse the sample collection tube and transfer 2 drops of the extracted sample (approximately 90 μl) to the sample cup (S) of the detection device and start the stopwatch. Avoid transferring air bubbles into the sample cup (S).

5. Wait until the coloured lines appear. Read the results after 5 minutes. Do not interpret the result after 10 minutes.

Interpretation of the results:
– POSITIVE:* Two different coloured lines appear. One line should appear in the control line area (C) and another in the test line area (T).
NOTE: The colour intensity of the test line area (T) will vary depending on the concentration of occult blood in faeces present in the sample. Therefore, any colour tone in the detection line area (T) should be considered positive.
– NEGATIVE: A coloured line appears in the control line area (C). No apparent coloured line appears in the test line area (T).
– INCORRECT: The control line does not appear. Insufficient sample volume or incorrect procedure technique are often the main reasons for invalidating the test. Check the procedure and repeat the test with a new device. If the problem persists, immediately discontinue use of the test kit and contact your local distributor.